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celltrace violet cell proliferation kit 15579992  (Fisher Scientific)

 
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    Fisher Scientific celltrace violet cell proliferation kit 15579992
    Celltrace Violet Cell Proliferation Kit 15579992, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+violet+cell+proliferation+kit/celltrace+violet+proliferation+dye/bio_rxiv__2025__04__16__649059-277-21-27
    Average 90 stars, based on 1 article reviews
    celltrace violet cell proliferation kit 15579992 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: TMEM244 Gene Expression as a Potential Blood Diagnostic Marker Distinguishing Sézary Syndrome from Mycosis Fungoides and Benign Erythroderma.
    Article Snippet: Cell doubling time was determined using the CellTrace violet Cell proliferation Kit (Fisher Scientific) according to the manufacturer’s instructions. .. B

    Article Title: Bone marrow MSC from pediatric patients with B-ALL highly immunosuppress T-cell responses but do not compromise CD19-CAR T-cell activity
    Article Snippet: was purchased from Jazz Pharmaceuticals Iberia SL, Barcelona, Spain. .. CellTrace Violet Cell Proliferation Kit and CellTrace CFSE Cell Proliferation Kit were purchased from Fisher Scientific SL, Madrid, Spain. .. Cell fixation and permeabilization Kit was purchased from Nordic-Mubio, Derio, Spai

    Labeling:

    Article Title: TMEM244 Gene Expression as a Potential Blood Diagnostic Marker Distinguishing Sézary Syndrome from Mycosis Fungoides and Benign Erythroderma.
    Article Snippet: Cell doubling time was determined using the CellTrace violet Cell proliferation Kit (Fisher Scientific) according to the manufacturer’s instructions. .. B

    Article Title: Bone marrow MSC from pediatric patients with B-ALL highly immunosuppress T-cell responses but do not compromise CD19-CAR T-cell activity
    Article Snippet: was purchased from Jazz Pharmaceuticals Iberia SL, Barcelona, Spain. .. CellTrace Violet Cell Proliferation Kit and CellTrace CFSE Cell Proliferation Kit were purchased from Fisher Scientific SL, Madrid, Spain. .. Cell fixation and permeabilization Kit was purchased from Nordic-Mubio, Derio, Spai

    Cell Culture:

    Article Title: TMEM244 Gene Expression as a Potential Blood Diagnostic Marker Distinguishing Sézary Syndrome from Mycosis Fungoides and Benign Erythroderma.
    Article Snippet: Cell doubling time was determined using the CellTrace violet Cell proliferation Kit (Fisher Scientific) according to the manufacturer’s instructions. .. B

    Article Title: Bone marrow MSC from pediatric patients with B-ALL highly immunosuppress T-cell responses but do not compromise CD19-CAR T-cell activity
    Article Snippet: was purchased from Jazz Pharmaceuticals Iberia SL, Barcelona, Spain. .. CellTrace Violet Cell Proliferation Kit and CellTrace CFSE Cell Proliferation Kit were purchased from Fisher Scientific SL, Madrid, Spain. .. Cell fixation and permeabilization Kit was purchased from Nordic-Mubio, Derio, Spai



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    (A) Donor H2 Ag in splenic FDCs (inset, arrows) after transplantation of B6 (H2 b ) hearts in BALB/c mice. Original magnification ×200. Scale bars, 20 μm. (B) Quantification with ImageJ of donor H2 Ag spots per FDC network on spleens of BALB/c mice transplanted with B6 hearts. (C) Donor H2 Ag spots per FDC network on BALB/c LNs draining B6 skin allografts assessed using ImageJ. (D) Deep SIM of donor H2 Ag spots in FDCs of a BALB/c LN draining a B6 skin graft. Scale bar, 2 μm. ROI, region of interest. (E and F) 2P-microscopy of FDCs in the spleen of a BALB/c mouse transplanted with a B6 heart releasing RFP-sEVs (E) and a BALB/c LN draining a B6 skin graft releasing RFP-sEVs (F). Arrows: graft-RFP-sEVs on the surface (red) or inside (yellow) of FDCs (green). <t>CellTrace</t> Violet-B cells indicate B cell follicles. FDCs were labeled with AF488-CD21/CD35 Ab. Original magnification ×25. Scale bars, 20 μm. (G) Colocalization by confocal microscopy of donor (B6, IA b + H2K b ) allo-Ags in FDCs of LNs draining CMV Cre/+ LSL -RFP-CD63 B6 skin grafted in BALB/c mice. Scale bar, 10 μm. Mander’s colocalization coefficient of graft-sEVs and donor IA b + H2K b Ab analyzed using Imaris. Each triangle represents one FDC network. In (E) and (F), RFP spots per FDC network z stacks were quantified within 90-μm-thick z stacks using Imaris. Experiments were done on 4 spleens per POD (A and B), 6 graft-dLNs per POD (C), 3 graft-dLNs (D), 4 spleens (E), 4 graft-dLNs (F), and 12 graft-dLNs (G). In (B), (C), (E), and (F), comparisons were made by multiple unpaired two-tailed Student’s test. Error bars, means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; NS, not significant.
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    (A) Donor H2 Ag in splenic FDCs (inset, arrows) after transplantation of B6 (H2 b ) hearts in BALB/c mice. Original magnification ×200. Scale bars, 20 μm. (B) Quantification with ImageJ of donor H2 Ag spots per FDC network on spleens of BALB/c mice transplanted with B6 hearts. (C) Donor H2 Ag spots per FDC network on BALB/c LNs draining B6 skin allografts assessed using ImageJ. (D) Deep SIM of donor H2 Ag spots in FDCs of a BALB/c LN draining a B6 skin graft. Scale bar, 2 μm. ROI, region of interest. (E and F) 2P-microscopy of FDCs in the spleen of a BALB/c mouse transplanted with a B6 heart releasing RFP-sEVs (E) and a BALB/c LN draining a B6 skin graft releasing RFP-sEVs (F). Arrows: graft-RFP-sEVs on the surface (red) or inside (yellow) of FDCs (green). <t>CellTrace</t> Violet-B cells indicate B cell follicles. FDCs were labeled with AF488-CD21/CD35 Ab. Original magnification ×25. Scale bars, 20 μm. (G) Colocalization by confocal microscopy of donor (B6, IA b + H2K b ) allo-Ags in FDCs of LNs draining CMV Cre/+ LSL -RFP-CD63 B6 skin grafted in BALB/c mice. Scale bar, 10 μm. Mander’s colocalization coefficient of graft-sEVs and donor IA b + H2K b Ab analyzed using Imaris. Each triangle represents one FDC network. In (E) and (F), RFP spots per FDC network z stacks were quantified within 90-μm-thick z stacks using Imaris. Experiments were done on 4 spleens per POD (A and B), 6 graft-dLNs per POD (C), 3 graft-dLNs (D), 4 spleens (E), 4 graft-dLNs (F), and 12 graft-dLNs (G). In (B), (C), (E), and (F), comparisons were made by multiple unpaired two-tailed Student’s test. Error bars, means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; NS, not significant.
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    Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with <t>CellTrace</t> Violet <t>(CTV)</t> and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and <t>proliferation</t> and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).
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    Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with <t>CellTrace</t> Violet <t>(CTV)</t> and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and <t>proliferation</t> and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).
    Celltrace Violet Cell Proliferation Kit 15579992, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+violet+cell+proliferation+kit/celltrace+violet+proliferation+dye/bio_rxiv__2025__04__16__649059-277-21-27
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    (A) Donor H2 Ag in splenic FDCs (inset, arrows) after transplantation of B6 (H2 b ) hearts in BALB/c mice. Original magnification ×200. Scale bars, 20 μm. (B) Quantification with ImageJ of donor H2 Ag spots per FDC network on spleens of BALB/c mice transplanted with B6 hearts. (C) Donor H2 Ag spots per FDC network on BALB/c LNs draining B6 skin allografts assessed using ImageJ. (D) Deep SIM of donor H2 Ag spots in FDCs of a BALB/c LN draining a B6 skin graft. Scale bar, 2 μm. ROI, region of interest. (E and F) 2P-microscopy of FDCs in the spleen of a BALB/c mouse transplanted with a B6 heart releasing RFP-sEVs (E) and a BALB/c LN draining a B6 skin graft releasing RFP-sEVs (F). Arrows: graft-RFP-sEVs on the surface (red) or inside (yellow) of FDCs (green). CellTrace Violet-B cells indicate B cell follicles. FDCs were labeled with AF488-CD21/CD35 Ab. Original magnification ×25. Scale bars, 20 μm. (G) Colocalization by confocal microscopy of donor (B6, IA b + H2K b ) allo-Ags in FDCs of LNs draining CMV Cre/+ LSL -RFP-CD63 B6 skin grafted in BALB/c mice. Scale bar, 10 μm. Mander’s colocalization coefficient of graft-sEVs and donor IA b + H2K b Ab analyzed using Imaris. Each triangle represents one FDC network. In (E) and (F), RFP spots per FDC network z stacks were quantified within 90-μm-thick z stacks using Imaris. Experiments were done on 4 spleens per POD (A and B), 6 graft-dLNs per POD (C), 3 graft-dLNs (D), 4 spleens (E), 4 graft-dLNs (F), and 12 graft-dLNs (G). In (B), (C), (E), and (F), comparisons were made by multiple unpaired two-tailed Student’s test. Error bars, means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; NS, not significant.

    Journal: Cell reports

    Article Title: Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells

    doi: 10.1016/j.celrep.2025.115832

    Figure Lengend Snippet: (A) Donor H2 Ag in splenic FDCs (inset, arrows) after transplantation of B6 (H2 b ) hearts in BALB/c mice. Original magnification ×200. Scale bars, 20 μm. (B) Quantification with ImageJ of donor H2 Ag spots per FDC network on spleens of BALB/c mice transplanted with B6 hearts. (C) Donor H2 Ag spots per FDC network on BALB/c LNs draining B6 skin allografts assessed using ImageJ. (D) Deep SIM of donor H2 Ag spots in FDCs of a BALB/c LN draining a B6 skin graft. Scale bar, 2 μm. ROI, region of interest. (E and F) 2P-microscopy of FDCs in the spleen of a BALB/c mouse transplanted with a B6 heart releasing RFP-sEVs (E) and a BALB/c LN draining a B6 skin graft releasing RFP-sEVs (F). Arrows: graft-RFP-sEVs on the surface (red) or inside (yellow) of FDCs (green). CellTrace Violet-B cells indicate B cell follicles. FDCs were labeled with AF488-CD21/CD35 Ab. Original magnification ×25. Scale bars, 20 μm. (G) Colocalization by confocal microscopy of donor (B6, IA b + H2K b ) allo-Ags in FDCs of LNs draining CMV Cre/+ LSL -RFP-CD63 B6 skin grafted in BALB/c mice. Scale bar, 10 μm. Mander’s colocalization coefficient of graft-sEVs and donor IA b + H2K b Ab analyzed using Imaris. Each triangle represents one FDC network. In (E) and (F), RFP spots per FDC network z stacks were quantified within 90-μm-thick z stacks using Imaris. Experiments were done on 4 spleens per POD (A and B), 6 graft-dLNs per POD (C), 3 graft-dLNs (D), 4 spleens (E), 4 graft-dLNs (F), and 12 graft-dLNs (G). In (B), (C), (E), and (F), comparisons were made by multiple unpaired two-tailed Student’s test. Error bars, means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; NS, not significant.

    Article Snippet: CellTrace TM Violet Cell Proliferation Kit, for flow cytometry , Thermo Fisher , Cat # C34557.

    Techniques: Transplantation Assay, Microscopy, Labeling, Confocal Microscopy, Two Tailed Test

    Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with CellTrace Violet (CTV) and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).

    Journal: Drug Delivery

    Article Title: Selective expansion and differentiation of antigen-specific CD4 + T-helper cells by engineered extracellular vesicles

    doi: 10.1080/10717544.2025.2509969

    Figure Lengend Snippet: Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with CellTrace Violet (CTV) and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).

    Article Snippet: Lymph node T cells isolated from C57BL/6 and OT-II transgenic mice were stained with 1 μM CellTrace Violet (CTV) Cell Proliferation Kit (Thermo Fisher Scientific, Waltham, MA) for flow cytometry and incubated at 37 °C for 3 min.

    Techniques: In Vitro, Transgenic Assay, Labeling, Control, Cell Culture, Flow Cytometry, Expressing